Journal: Nature Communications
Article Title: Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces
doi: 10.1038/s41467-024-53542-5
Figure Lengend Snippet: A Experimental procedure for the proteomic investigation ( n = 3 for each tissue). B – E Tests for differential expression of proteins were based on protein-wise linear models and empirical Bayes statistics . The plots are based on the fold change (log 2 ) and the P -value (-log 10 ) of all proteins identified in ( B ) proteins from FE and FL; ( C ) proteins from FE and FB; ( D ) proteins from TE and TL; ( E ) proteins from TE and TB. F Immunofluorescence staining of SLC25A1 (cyan) and CLEC11A (magenta) in ACL-femur tissues and SRPX (cyan) and MGP (magenta) in ACL-tibia tissues. Scale bar: 20 μm. Abbreviations, FL, the femoral part of ACL; FE, ACL-femoral enthesis; FB, ACL-femoral subchondral bone; TL, the tibial part of ACL; TE, ACL-tibial enthesis; TB, ACL-tibial subchondral bone.
Article Snippet: Next, the femoral enthesis samples were subjected to incubation with various primary antibodies, including SLC25A12 antibody (1:50, 67467-1-Ig, Proteintech) and CLEC11A antibody (1:100, 55019-1-AP, Proteintech).
Techniques: Quantitative Proteomics, Immunofluorescence, Staining